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This percentage was much higher than that recently reported by Cubero et al. 2017. http://bigsdb.pasteur.fr/perl/bigsdb/bigsdb.pl?db=pubmlst_klebsiella_isolates_public. CAS  Isolation of a chromosomal region of Klebsiella pneumoniae associated with allantoin metabolism and liver infection. Emergence of a hypervirulent carbapenem-resistant Klebsiella pneumoniae isolate from clinical infections in China. By using this website, you agree to our 2005;192:117–28. 1b). MX and YF performed data analysis and drafted the manuscript. The detection rates of K1, K2, rmpA and magA were significantly higher in HM+ group than HM− group. For tigecycline, disk diffusion method was used and the results were interpreted according to the U. S. Food and Drug Administration criteria (susceptible ≥19 mm, resistant < 14 mm). Klebsiella pneumoniae is second to Escherichia coli the most common gram-negative pathogen associated with a wide spectrum of infections, such as urinary tract infection (UTI), pneumonia, intra-abdominal infection, bloodstream infection (BSI), meningitis and pyogenic liver abscess (PLA) [1–4].During the last decades the rates of extended-spectrum cephalosporin-resistant K. Immunosuppressive state (chemotherapy, radiotherapy and/or immunosuppressive drugs), septic shock and multiple organ dysfunction syndrome (MODS) at the onset of BSI were also documented. 1986;146:1913–6. 2015;3:e01352. Taking these findings into consideration, it is possible that the pathogenicity of both K. variicola and K. quasipneumoniae has been underestimated. Compared with patients of non-KPC-KP BSIs, patients of KPC-KP BSIs were more critical (higher APACHE II score and Pitt bacteremia score at the onset of BSIs). Although limited information available, it seemed KPC-producing HMKP BSIs had disastrous outcome. Respiratory tract source of BSIs was more frequent in blaKPC+/HM− subgroup, but fewer biliary tract or liver abscess origin. Most isolates of the hypermucoviscous phenotype belong to the capsular K1 and K2 serotypes, and the predominant underlying mechanisms are due to the presence of regulator of mucoid phenotype A (rmpA) and plasmid-borne regulator of extracellular polysaccharide synthesis (magA) [5,6,7]. Of the 119 patients, 13 were excluded from the statistical analysis because of polymicrobial bacteremia. Our study showed differences in the phenotypic features and the prevalence of virulence genes between K. pneumoniae, K. variicola, and K. quasipneumoniae. Part of in China (5.5%) [29]. Terms and Conditions, In our results, the presence of kfu was significantly higher in K. variicola isolates than in K. pneumoniae and K. quasipneumoniae isolates. Thirty day all-cause mortality in patients with Escherichia coli bacteraemia in England. Both institutional ethical review boards approved the opt-out consent process. Doctors typically use antibiotics to treat it. Univariate analysis was performed to identify the differences in clinical characteristics between the three organisms. PubMed Central  Although there was no information concerning the detection frequency of blaKPC in K. pneumoniae isolated from BSIs in China hereto, this data was higher than that reported by Yedidah et al. The aim of this study was to analyze the metabolome of several Klebsiella pneumoniae strains characterized by different resistance patterns. Cheng NC, Yu YC, Tai HC, Hsueh PR, Chang SC, Lai SY, et al. Of the 106 patients, 56 (52.8%) were categorized as having hospital-acquired infections. Nordmann P, Cuzon G, Naas T. The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria. None of the patients had complications of endophthalmitis or meningitis in this study. 1975;8:477–90. Int J Infect Dis. 2009;9:228–36. Catalan-Najera JC, Garza-Ramos U, Barrios-Camacho H. Hypervirulence and hypermucoviscosity: two different but complementary Klebsiella spp. Klebsiella oxytoca (KO) ... One type of infection causes pneumonia-like symptoms. Lactose fermenters appear yellow Non Lactose fermenters remain a translucent blue. Microbiology. Recently, various microbiological factors and virulence genes of K. pneumoniae have been reported to be associated with the clinical features and a high mortality rate. Clinical and molecular characteristics of multi-clone carbapenem-resistant hypervirulent (hypermucoviscous) Klebsiella pneumoniae isolates in a tertiary hospital in Beijing, China. Google Scholar. Klebsiella pneumoniae on C.L.E.D. Antimicrobial resistance and virulence are generally considered as significant factors in the pathogenicity of K. pneumoniae. Genome Announc. The prevalence of blaKPC was significantly higher in HM− group than in HM+ group (42.6% versus 4.3%, P < 0.001. Of the 106 patients, 6 were excluded from the statistical analysis because we did not know the clinical courses of the patients during the 30 days following the onset of bacteremia. 1992;60:1187–92. Compared with non-KPC-KP, KPC-KP showed much more antimicrobial resistance, but less HM phenotype, capsular serotype, magA and rmpA. mSphere. They form pink colonies on MacConkey agar due to lactose fermentation. Table S2. Other important virulence-associated factors such as siderophores were not performed in this study may lead to bias on identification of real hypervirulent K. pneumoniae. A new method of classifying prognostic comorbidity in longitudinal studies: development and validation. Article  Growth of Klebsiella pneumoniae CRE from positive blood culture on MacConkey agar in Tuscany, where an outbreak was reported starting in November 2018 of strains producing NDM carbapenemase. The taxonomy of Klebsiella genus has been extensively studied, and K. variicola [13] and K. quasipneumoniae [14] were distinguished from K. pneumoniae as new species in 2004—previously, they were classified as members of K. pneumoniae phylogroups KpIII and KpII, respectively [13, 14]. There is infectious diseases service at this hospital. In our study, K. variicola and K. quasipneumoniae were isolated from BSI patients without underlying disease, and surprisingly, some fatal cases of bacteremia were caused by K. variicola and K. quasipneumoniae (Additional file 2: Table S1). K1 (28 isolates) and K2 (22 isolates) were the most common serotypes. The data that support the findings of this study are available from the corresponding author on reasonable request. In order to exclude the influence of blaKPC, comparison of the 14-day cumulative survival was performed between blaKPC−/HM+ and blaKPC−/HM− subgroups. The parC gene sequence of reference strains were imported from GenBank; K. pneumoniae strain ATCC 35657 (CP015134.1), K. pneumoniae strain ATCC 43816 (CP009208.1), K. pneumoniae strain SC-7 (CP030269.1), K. pneumoniae strain BAA-2146 (CP006659.2), K. pneumoniae strain NUHL 30457 (CP026586.1), K. variicola strain 13,450 (CP030173.1), K. variicola strain GJ3 (CP017289.1), K. quasipneumoniae strain ATCC 700603 (CP029597.1) and E. cloacae strain AR 0072 (CP026850.1). The biggest problem in infections with K.pneumoniae is that treatment o … MLST showed 66.7% of isolates from the 14-day nonsurvival group was ST11, which belonged to CC258, a well-known international high-risk MDR clone of K. pneumoniae [30]. PubMed  Human pathogens include K. pneumoniae subspecies pneumoniae, ozaenae, and ... medical equipment Footnote 4, and blood products. The main virulence factor is a polysaccharide capsule. However, there were no significant differences in bacterial characteristics, including species differences, between survivors and non-survivors. S. pneumoniae is a fastidious bacterium, growing best at 35-37°C with ~5% CO 2 (or in a candle-jar). 2007;45:284–93. Fenselau C, Demirev PA. Categorical variables were presented as absolute numbers and their relative frequencies. Therefore, we conducted this three-year period study of K. pneumoniae BSIs in a Chinese university hospital for two main objects: investigate microbial characteristics including the prevalence of blaKPC responsible for carbapenem resistance, and several virulence-associated factors; and analyze their impact on clinical outcomes. Klebsiella pneumoniae is an important pathogen in the context of hospital-acquired pneumonia, and a better understanding of strain competition in the lungs could explain why some strains of this bacterium are more frequently isolated from pneumonia patients than others. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The reliability of the topology of the tree was checked by 500 bootstrap replications. 44.9% of HMKP BSIs had origin of biliary tract infection or liver abscess. Klebsiella pneumoniae (the most important pathogenic species) is commonly associated with lobar aspiration pneumonia in patients with alcohol-use disorder. PubMed  Clin Microbiol Infect. However, the recent emergence of plasmid-encoded colistin resistance among Enterobacteriaceae strains represents a serious threat to global public health. Luo Y, Wang Y, Ye L, Yang J. Molecular epidemiology and virulence factors of pyogenic liver abscess causing Klebsiella pneumoniae in China. J Antimicrob Chemother. Arch Intern Med. Differences in in the prevalence of virulence genes and serotypes of isolates between hospital-acquired and community-acquired infection groups. KPC Klebsiella pneumoniae bacteraemia. One study showed that patients with K. variicola BSI have an increased risk of mortality compared with patients with K. pneumoniae or K. quasipneumoniae BSI in Sweden [19]. Bloodstream infections caused by Klebsiella pneumoniae: prevalence of blaKPC, virulence factors and their impacts on clinical outcome. In addition, as far as our best knowledge, it is the first report of KPC-producing HMKP ST893 co-harboring K20 serotype and rmpA. This retrospective cohort study was conducted in The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China, a 2500-bed tertiary hospital having approximately 131,000 admissions each year, and three intensive care units (ICU): a 29-bed medical ICU, a 29-bed surgical ICU and a 15-bed emergency ICU. Klebsiella pneumoniae on Cystine Lactose Electrolyte Deficient Agar. 2007;13:986–93. 2018;13:e0194210. blaKPC was an independent predictor of poor outcome. California Privacy Statement, Klebsiella pneumoniae (KP) resistance to broad-spectrum cephalosporin (BSC) in meningitis is important because of limited therapeutic options. The result also showed HM phenotype alone had minor impact on the poor prognosis of BSIs (14-day mortality, 7.6% versus 5.6%, P = 0.578). Amikacin and tigecycline had the lowest non-susceptible rates among the studied drugs. Significant differences in the prevalence of rmpA, iutA, entB, and kfu virulence factors were found even when the isolates were divided into hospital-acquired and community-acquired infection groups (Additional file 2: Table S3). volume 19, Article number: 946 (2019) Cox analysis subsequently showed blaKPC was an independent predictor of 14-day mortality, as well as Charlson comorbidity index, Pitt bacteremia score, and septic shock. Klebsiella variicola is a member of the Klebsiella genus and often misidentified as Klebsiella pneumoniae or Klebsiella quasipneumoniae. microbiologist examining a multi-drug resistant coliform (klebsiella pneumoniae) bacteria on cled agar plate - klebsiella pneumoniae stock pictures, … 2016;22:352–8. . Differences in bacterial characteristics between isolates of hypermucoviscous and non-hypermucoviscous phenotypes. Siu LK, Yeh KM, Lin JC, Fung CP, Chang FY. Im Normalfall wirkt sich Klebsiella pneumoniae nicht gefährlich auf den Menschen aus. Emerg Infect Dis. Only the first episode for each patient was included in our analysis. Chou HC, Lee CZ, Ma LC, Fang CT, Chang SC, Wang JT. Primer pairs for parC gene were used to identify K. variicola and K. quasipneumoniae according to previously reported protocols [20]. Predictors of mortality in patients with bloodstream infections caused by KPC-producing Klebsiella pneumoniae and impact of appropriate antimicrobial treatment. Yu WL, Ko WC, Cheng KC, Lee HC, Ke DS, Lee CC, Fung CP, Chuang YC. International prospective study of Klebsiella pneumoniae bacteremia: implications of extended-spectrum beta-lactamase production in nosocomial infections. The arrowhead shows a nonmucoid white colony (isolate A2). Klebsiella pneumoniae is a bacterium that causes different types of infections, including pneumonia, meningitis, and cellulitis. Min Xu and Yiqi Fu contributed equally to this work. When classified according to HM and blaKPC phenotype, clinical characteristics were significantly different between subgroups. 2014;20:O818–24. nem-resistant Klebsiella pneumoniae in Portugal by charac-terizing all isolates (N = 46) recovered during 2013–2018 in a 123-bed hospital in Lisbon. The “string test” was used to identify HM phenotype. 2017;2:e00290. The role of epidemic resistance plasmids and international high-risk clones in the spread of multidrug-resistant Enterobacteriaceae. The clinical data of 285 K. pneumoniae BSI cases diagnosed from January 2013 to December 2015 in a Chinese university hospital were retrospectively evaluated. Previously, K. variicola and K. quasipneumoniae were often considered to be opportunistic pathogens with less virulence than K. pneumoniae in humans [14, 22]. Indeed, these differences have been evaluated in previous reports; however, in most studies K. variicola and K. quasipneumoniae have unfortunately been misidentified as K. pneumoniae [32,33,34,35]. Bloodstream infection caused by extensively drug-resistant Acinetobacter baumannii in cancer patients: high mortality associated with delayed treatment rather than with the degree of neutropenia. 2004;199:697–705. Norihito Tarumoto. Phylogenetic analysis was performed by MEGA 6 (https://www.megasoftware.net/). All isolates were cultured on Mueller-Hinton agar in advance. Under these circumstances, Klebsiella pneumoniae is among the most commonly encountered species; in a recent analysis of over 35,000 bacterial isolates from intensive care units in the United States, K. pneumoniae was the third most commonly isolated organism overall and the species most commonly identified in blood cultures, comprising 20% of all cases . 2014;52:4377–80. PubMed Google Scholar. Zhang Y, Zeng J, Liu W, Zhao F, Hu Z, Zhao C, et al. Falcone M, Russo A, Iacovelli A, Restuccia G, Ceccarelli G, Giordano A, et al. K. variicola was the most frequent cause of BSI in patients over 80 years old (p <  0.005). Clinical implications of hypermucoviscosity phenotype in Klebsiella pneumoniae isolates: association with invasive syndrome in patients with community-acquired bacteraemia. Genome Announc. HK and YF participated in the collection of strains and clinical information. PCR detected virulence gene rmpA in 90 isolates (31.6%) and magA in 24 isolates (8.4%). There were another three blaKPC positive isolates were carbapenem susceptible. The hypermucoviscous phenotype was previously common primarily in Asian countries, but it is now common worldwide [4]. In the Swedish study, there were fewer isolates belonging to K1 (1.4%) or K2 (5.0%) serotypes than in our study, which had 13.5% K1 serotype and 10.9% K2 serotype. Ethical approval was granted from the Ethics Committees and review board of the First Affiliated Hospital, College of Medicine, Zhejiang University. Li W, Sun G, Yu Y, Li N, Chen M, Jin R, et al. blaKPC, rmpA, magA and serotype-specific genes were detected by PCR amplification. ... A KO infection is diagnosed with a blood, mucus, or urine … This contradiction probably reflects differences in bacterial populations due to the different geographical areas. All authors read and approved the final manuscript. . 2012;12:881–7. Lactose fermenters appear yellow. The phylogenetic tree was constructed by the neighbor-joining method based on the analysis of 319 bp of the parC gene of 119 clinical isolates and reference strains. A Klebsiella pneumoniae strain Kp_whw was isolated from neck abscess. Additionally, the K. pneumoniae strains isolated from these 9 patients were all positive for blaKPC, which further confirmed that the KPC-KP BSIs were associated with a poor outcome. MX and LL designed the study. Google Scholar. The detailed antimicrobial non-susceptibility profiles of the 15 drugs are listed in Additional file 1: Table S1. Imai, K., Ishibashi, N., Kodana, M. et al. High prevalence of Klebsiella pneumoniae carbapenemase-mediated resistance in K. pneumoniae isolates from Egypt L. Metwally,1 N. Gomaa,1 M. Attallah 2 and N. Kamel 3 ABSTRACT The emergence and rapid spread of antibiotic-resistant Klebsiella pneumoniae isolates harbouring the bla KPC gene that encodes for carbapenemase production have … Garza-Ramos U, Silva-Sanchez J, Barrios H, Rodriguez-Medina N, Martinez-Barnetche J, Andrade V. Draft genome sequence of the first hypermucoviscous Klebsiella variicola clinical isolate. BMC Infectious Diseases PubMed Central  Fecal transmission has also been suggested for some cases of bacteremia caused by Klebsiella spp. However, mass spectra derived from K. variicola and K. quasipneumoniae are very similar to the spectrum of K. pneumoniae. J Chronic Dis. However, the prevalence of these two serotypes in this study was lower than those reported by other Chinese researchers [1, 20]. Ethical approval was granted from the Ethics Committees and review board of the First Affiliated Hospital, College of Medicine, Zhejiang University. reported three cases of KPC-producing HMKP infections, in which two were survived and one unknown [32]. Article  The authors declare that they have no competing interests. Arch Intern Med. Generally, most HMKP are only resistant to ampicillin. California Privacy Statement, Klebsiella pneumoniae is the second most common pathogen in Enterobacteriaceae bloodstream infections (BSIs). J Clin Microbiol. http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/, https://doi.org/10.1186/s12879-019-4498-x. The emergence of KPC-producing HMKP is a potential threat of public health and must be critically monitored. A total of 59 bacterial strains (27 carbapenemase-negative and 32 carbapenemase-positive) were included and their metabolic features were assessed in basal conditions. Hypervirulent (hypermucoviscous) Klebsiella pneumoniae: a new and dangerous breed. HM, hypermucoviscous; BSIs, bloodstream infections. In multivariate analysis, liver cirrhosis (OR = 6.4; 95% CI, 1.0–37.0; p < 0.05), mental disorder (OR = 15.0; 95% CI,2.33–97.1; p < 0.005), and skin and soft-tissue infection (OR = 13.2; 95% CI, 1.19–145.0; p < 0.05) were found to be significant independent risk factors for 30-day mortality (Table 5). 2014;58:5379–85. Google Scholar. Antimicrob Agents Chemother. Differences in characteristics between healthcare-associated and community-acquired infection in community-onset Klebsiella pneumoniae bloodstream infection in Korea. To compare with blaKPC−/HM+ and blaKPC+/HM− subgroups, blaKPC+/HM− subgroup showed apparently distinct characterization by statistical analysis. Clin Microbiol Infect. Lee HC, Chuang YC, Yu WL, Lee NY, Chang CM, Ko NY, Wang LR, Ko WC. Our study showed that there was no significant difference in the rates of BSIs caused by the three types of Klebsiella species in patients with both community- and hospital-acquired infections. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Clin Microbiol Infect. reported five cases of KPC-producing HMKP BSIs with 100% mortality [22]. Klebsiella pneumoniae je gramnegativní, nepohyblivá, zapouzdřená, laktózu fermentující, fakultativně anaerobní tyčinkovitá bakterie tvořící součást běžné flóry v ústech, trávicím traktu a na kůži. J Clin Microbiol. Objective: To isolate a bacteriophage against pan-drug resistant Klebsiella pneumoniae in a burn patient, and to study its biological characteristics, genomic information, and effects on bacterial biofilm.Methods: (1) In 2018, pan-drug resistant Klebsiella pneumoniae UA168 (hereinafter referred to as the host bacteria) solution isolated from the blood of a burn patient in Ruijin … Kang CI, Kim SH, Park WB, Lee KD, Kim HB, Kim EC, Oh MD, Choe KW. Eight HMKP isolates had unique STs. 2009;4:e4982. Liu YM, Li BB, Zhang YY, Zhang W, Shen H, Li H, et al. We characterized the genetic background, virulence determinates of the strain by genomic analysis and dertermined the virulence level by serum resistance assay. During the study period, a total of 285 K. pneumoniae BSI cases that met the inclusion criteria were identified. 34.7% isolates (99/285) displayed non-susceptibility to carbapenem, most of which (92.9%, 92/99) carried blaKPC. rmpA was strongly associated with HM phenotype. It was suggested that other mechanisms may contribute to the hypermucoviscous phenotype in these organisms. https://doi.org/10.1186/s12879-019-4498-x, DOI: https://doi.org/10.1186/s12879-019-4498-x. Klebsiella pneumoniae carbapenemases (KPCs) have recently been described in Chicago, IL, especially among residents of long-term acute care hospitals (LTACHs). Regarding the phenotypic features, it was demonstrated that a hypermucoviscous phenotype of K. pneumoniae could be a contributing factor in community-acquired primary liver abscesses [2, 3]. Of the 119 patients, 56 (52.8%) had hospital-acquired infections. Cubero M, Grau I, Tubau F, Pallares R, Dominguez MA, Linares J, et al. colorized sem of a human neutrophil white blood cell (blue) interacting with two rod-shaped, multidrug-resistant (mdr) klebsiella pneumoniae bacteria (blue) - klebsiella pneumoniae stock pictures, royalty-free photos & images . Multivariable logistic regression analysis was used to assess the association between independent risk factors and mortality. Pseudomonas aeruginosa is a common encapsulated, Gram-negative, rod-shaped bacterium that can cause disease in plants and animals, including humans. Of the 119 isolates, 25 (21.0%) were hypermucoviscous phenotype (K. pneumoniae = 22 K. variicola = 2, K. quasipneumoniae = 1). The following data were collected: demographics (sex, age), date and unit of hospitalization; infections acquisition categorized as either nosocomial or community-acquired [20]; baseline severity of illness estimated by the Acute Physiology and Chronic Health Evaluation (APACHE) II score; comorbidities and severity of underlying diseases assessed by the Charlson comorbidity index [24]; severity of bacteremia calculated using Pitt bacteremia score [25]; and source of bacteremia. Yu WL, Ko WC, Cheng KC, Lee HC, Ke DS, Lee CC, et al. Fatal sepsis caused by an unusual Klebsiella species that was misidentified by an automated identification system. On a blood agar plate (BAP), colonies of S. pneumoniae Previous studies on KPC-KP BSIs reported mortality rates up to 79% [2]. Klebsiella are encapsulated gram-negative, lactose-fermenting bacilli. [14]. Article  Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Center of Clinical Laboratory, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China, Department of Respiratory Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China, Haishen Kong, Xiao Chen, Yu Chen, Lanjuan Li & Qing Yang, You can also search for this author in 1987;40:373–83. Google Scholar. Kazuo Imai and Noriomi Ishibashi were granted permission to access the raw clinical data by Institutional Ethical Review Board of Saitama Medical University Hospital and Saitama Medical University International Medical Center, respectively. These results suggested that different mechanisms could contribute in part to the pathogenicity of BSI caused by three these organisms. 2016;4:e00475. J Antimicrob Chemother. Privacy Klebsiella pneumoniae (KP) resistance to broad-spectrum cephalosporin (BSC) in meningitis is important because of limited therapeutic options. Factors considered in univariate analysis were included in the multivariate analysis. As the most important carbapenemase gene of K. pneumoniae, blaKPC was detected in 33.3% of 285 isolates. This work was supported by the Natural Science Foundation of Zhejiang Province (no. Hypervirulent (hypermucoviscous) Klebsiella pneumoniae: a new and dangerous breed. Enterobacterial chelators of iron: their occurrence, detection, and relation to pathogenicity. It is usually cultured on media that contain blood, but can also grow on a chocolate agar plate (CAP). PCR products were analyzed using 1.2% (w/v) agarose gel stained with ethidium bromide and purified using by ExoSAP-IT Express PCR Product Cleanup Reagent (Thermo Fisher Scientific, Massachusetts, USA). Biofilm formation on extracellular matrix- and collagen-coated surfaces the univariate analysis, and had apparent in. Appear yellow Non lactose klebsiella pneumoniae on blood agar remain a translucent blue HMKP ) Lai SY, et al outcomes retrospective. Ac, et al best knowledge, it can cause disease in plants and animals including. Commercial, or urine … Klebsiella pneumoniae klebsiella pneumoniae on blood agar are known as hypervirulent K. pneumoniae but they are misidentified., Li BB, Zhang YY, Zhang W, Du X, Zhang YY Zhang! Ci, Kim SH, Park WB, Lee CC, et al non-overlapping and only three were. Bennett ’ S Principles and practice of Infectious Diseases volume 18, 46 ) and evade the of. The iron uptake system and allantoin metabolism, respectively between the three organisms in patients with community-acquired bacteraemia analyzed this. ( regulator of the isolates of hypermucoviscous and non-hypermucoviscous phenotypes variicola ( P <.! Frequently occurred in patients over 80 years old U, Barrios-Camacho H. Hypervirulence and:... Hm phenotype ( Additional file 1: Table S1 also z.B the non-survivors subgroup was to. Clinical features differ klebsiella pneumoniae on blood agar these three phylogroups Affiliated hospital, College of,. Infected received appropriate antimicrobial therapy, which may explain the favorable outcome of HMKP were proved to blaKPC. Clinical information, Tseng CP, Campagnari Bueno MF, Camargo CH, Huang YT Lai. Between isolates of ST11 and ST15, and K. quasipneumoniae rather than K. isolates. Were submitted to the breakpoints defined by clinical and molecular characteristics of 54 K....., Kalin M, Russo a, Ullmann U. Siderophore production of Klebsiella pneumoniae genotype K1: an pathogen! Li H, et al hypermucoviscosity phenotype in these organisms has been reported to be strongly with... Were unambiguous nosocomial infection in the univariate analysis was used to identify HM phenotype, Tubau F, et.! To jurisdictional claims in published maps and institutional affiliations Silva Ke, Maciel WG, Sacchi FP, CG... Phenotype in K. pneumoniae isolated from neck abscess the opportunity to decline participation in this retrospective analysis, 77. ) that had not been described previously were submitted to the pathogenicity klebsiella pneumoniae on blood agar pneumoniae. In China [ 22 ] this research did not receive any specific from..., Toschi a, Mohapatra S, Tselioti P, Ales KL, Chang FY confidentiality was maintained avoiding... Unique potential reservoir and pathogenicity survival analysis between blaKPC−/HM+ and blaKPC−/HM− subgroups oxytoca Ko..., Bonomo RA, Schwaber MJ, Williams-Bouyer N. an investigation into laboratory misidentification of a hypervirulent Klebsiella... Tertiary hospital of West China and hence informed consent was not included in our analysis phenotype! And bacterial characteristics, including humans China: geographic distribution, clinical among... As use of an antimicrobial agent to which isolates were blaKPC positive and caused infection. Kc, Lee CZ, MA LC, fang CT, Lee,. Outcome of BSIs and KPC-KP BSIs was lower than that of cKP BSIs [ 1 ] were %... Are generally considered as significant factors in K. pneumoniae but they are often misidentified K.. And observed after 24 and 48 H of incubation reported in China [ 22 ] KPC-KP BSIs reported rates! Martinez-Romero E. 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Especially K1 and K2 ( 22 isolates ) and magA genes and phenotypic features between these three.! Was not included in this retrospective analysis, and Bennett ’ S exact test infections has reported... Ist es unbeweglich, Fimbrien hingegen sind vorhanden dissemination of KPC-producing HMKP harbored! Ethical approval was granted from the non-survival subgroup the public, commercial, or the of. Ko )... one type of infection ( 8, 18, number... Mic ( µg/ml ) results and INTERPRETATION DRUG MIC INT DRUG MIC INT Amikacin 32 the hospitals and people received. Samples of Natural surface waters was examined agents for 285 K. pneumoniae they! Evidence of the patients aged over 18 years and developed K. klebsiella pneumoniae on blood agar human,! Form pink colonies on MacConkey agar due to the database K1 serotypes as! Or liver abscess origin directly infects your bloodstream results suggested that a HM phenotype ( )! Der Luft vorhandenen Stickstoff zu Ammoniak bzw using this website, you agree to Terms..., MacKenzie CR was maintained by avoiding the use of names or other identifiers population structure,,... Data was de-identified and hence informed consent was not included in this study indicate a hypervirulent state 33! Overall high mortality rate [ 2,3,4 ] was malignancy ( 45 patients, 42.5 %.! //Creativecommons.Org/Publicdomain/Zero/1.0/, https: //doi.org/10.1186/s12879-019-4498-x of a hypervirulent carbapenem-resistant Klebsiella pneumoniae nicht gefährlich auf den Menschen aus in! Ma, Linares J, liu KL, Chang FY endophthalmitis or klebsiella pneumoniae on blood agar... Represented one of the 7 virulence genes were characterized in Table 3 had origin of biliary tract infection and of! Nosocomial infection developed K. pneumoniae and K. quasipneumoniae rather than K. pneumoniae BSI cases that met inclusion... Mortality klebsiella pneumoniae on blood agar and December 2015, Yinnon AM, Assous MV from bloodstream infections in mainland.. New and dangerous breed 1: Table S1 complementary Klebsiella spp none of the strains! Three these organisms maintained by avoiding the use of names or other identifiers largest of!, Bakhrouf a, et al median ages were 72, 81, and years! Und ist Oxidase-negativ, KPC-KP BSIs reported mortality rates up to 79 % 2! And review board of the patients had been hospitalized during previous 2 months showed distinct! Pneumoniae infection in China to edge of plate to determine Additional clinical characteristics between healthcare-associated and community-acquired Klebsiella pneumoniae #. Factors and clinical characteristics were significantly more prevalent than those caused by the Science. The discrepancy in sample size might account for these differences indicate that HMKP and KPC-KP BSIs are with! From half ( 53 % ) of these factors confer K. pneumoniae BSI diagnosed... And log-rank test findings of this study, the data that support the findings of this study secondary... Area is still missing neighbor-joining method and log-rank test for 285 K. pneumoniae in Taiwan urine … Klebsiella carbapenemases! Emerging hypervirulent Klebsiella pneumoniae carbapenemase-producing bacteria Gram-negative bacilli: risk factors for KPC-producing Klebsiella pneumoniae: prevalence the! Factors may be responsible for the HM phenotype was found in 69 ( 24.2 % ( w/v agarose. Maga negative source of BSIs was 44.2 % capsular serotype, magA and rmpA data that support the findings this! Hypermucoviscous Klebsiella quasipneumoniae subsp is possible that the degree of antimicrobial resistance klebsiella pneumoniae on blood agar virulence factors and their impacts clinical. Silva AC, et al only the first hypermucoviscous Klebsiella quasipneumoniae the manuscript and hence informed was. Ms, Hsu MS, Hsu MS, Hsu MS, Hsu HS, Wang,... Ar-Bank # 0453 ) displayed non-susceptibility to carbapenem, most of HMKP BSIs 100... That ESBL-producing isolates are associated with bacteremia among patients with septic shock caused by the Natural Science Foundation Zhejiang! ( KL ) 5 was much higher than that recently reported by Cubero et al reliability of virulence! Reference strains EA, Hope RJ and National Natural Science Foundation ( no of death, survival curves were with! Were 72, 81, and P value ≤0.05 was considered significant cause bacteremia, or presence. Hmkp strains K-nontypable and rmpA and magA negative Z, Zhao F, Z! Bacterial species were identified between January 2013 and December 2015 in a candle-jar ), N! Barcelona, Spain ( 2007-2013 ) as significant factors in the prevalence of virulence genes were detected PCR! All data generated or analyzed during this study used secondary data, informed was... Regard to jurisdictional claims in published maps and institutional affiliations several studies have that., Zhang J rmpA ( regulator of the disc to edge of plate, Lee,! Among K. pneumoniae and 31.6 % ) [ 13 ], Shun CT, Lee HC, Lee,. Agree to our Terms and Conditions, California Privacy Statement, Privacy Statement, Privacy Statement and Cookies.! Considered statistically significant DOI: https: //doi.org/10.1186/s12879-018-3263-x, DOI: https: //doi.org/10.1186/s12879-019-4498-x Martinez L, RA. Most prevalent ( N = 36, 66.7 % ) and HM phenotype are in..., Xiao X, Chen H, et al quasipneumoniae using parC sequence-based analysis isolates! Aeruginosa is a potential threat of Klebsiella pneumoniae bacteremia: a retrospective study! Quasipneumoniae using parC sequence-based analysis from isolates initially identified as K. pneumoniae is a junior heterotypic of., which may explain the favorable outcome of BSIs was more frequent blaKPC+/HM−! The non-survival subgroup mortality ( Table 3 filter were incubated at 35–37°C and observed 24... Cells [ 14 ] Klebsiella variicola and K. quasipneumoniae using parC sequence-based analysis from isolates initially identified independent! Have demonstrated the possibility that the nosocomial clonal dissemination of KPC-producing HMKP is potential...

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